Mustard seed oil content and quality vary based on variety, climate, soil conditions, and harvest timing. Higher oil-content varieties provide better biodiesel yields. Drought stress or nutrient-poor soils can reduce both oil quantity and transesterification efficiency. The GlobeCore USB system helps mitigate input variability by optimizing reaction conditions and processing performance across different oil profiles. Selecting high-oil mustard cultivars improves both oil extraction and biodiesel economics.