Yes, olive oil can be used to produce biodiesel via transesterification. The process converts triglycerides in the oil into fatty acid methyl esters (FAMEs), commonly known as biodiesel, using methanol and a catalyst. Though more expensive than other oils, olive oil offers high-quality biodiesel with good ignition properties. The GlobeCore USB biodiesel plant ensures complete and clean conversion, even with varying oil grades, making olive oil a viable option—especially for small-scale or boutique biodiesel production.