The optimal reaction temperature is around 55–60°C, and the methanol-to-oil ratio should be about 6:1. Proper mixing at moderate speed (600–800 RPM) is critical for effective contact between oil, methanol, and catalyst. The GlobeCore USB plant is designed to maintain precise temperature control and optimal agitation, ensuring consistent reaction rates and complete transesterification. These conditions are ideal for coconut oil, which reacts faster due to its short-chain fatty acids, yielding a clean-burning biodiesel.